John Work may refer to:
- John Work (fur trader) (1792–1861), chief factor in Hudson's Bay Company and member of a founding family of Victoria, British Columbia
- John M. Work (1869–1961), American socialist and newspaper editor
- John Wesley Work, Jr. (1871–1925), American song collector and choral director
- John Wesley Work III (1901–1967), American composer and scholar, son of John Wesley Work, Jr.
- John Work House and Mill Site, historic gristmill in Charlestown, Indiana